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Specifically, this invention discloses a real-time TaqMan RT-PCR assay targeting the 5'-noncoding region of the HRV genome. Partek and Life Technologies have partnered to integrate Partek's bioinformatics software with Life Tech's real-time PCR assays, the companies said today. TaqMan® マルチプレックスリアルタイム ̶ - Life Technologies. Download Report PowerPoint TaqMan Mutation Detection Assay Detection of somatic mutations with competitive allele-specific real-time PCR and rel time PCR research services at the This preview shows page 78 - 80 out of 104 pages.. 1. Access the Custom TaqMan ® Assay Design Tool at . 2.
For more information, visit the Life Technologies booth (#4419) at the American Association of Cancer Research (AACR), March 31April 4 in Chicago, Ill. See show details here. The above referenced products are For Research Use Only. Applied Biosystems TaqMan SNP Genotyping assays help you detect and distinguish SNPs accurately and efficiently, so you can better understand their associations and roles in gene function and disease. Minor Groove Binder (MGB) technology enables TaqMan probes to discriminate between highly homologous allele sequences.
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Optimized TaqMan® probe and PCR primer sets: TaqMan® Assays are formulated for use right out of the box. Life Technologies scientists have developed the most comprehensive set of real-time PCR assays available for gene expression, microRNA, noncoding RNA, protein analysis, mutation detection, copy number variation, and SNP genotyping. TaqMan® SNP Genotyping Assays from Life Technologies provide a highly flexible technology for detection of polymorphisms within any genome.
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Article Snippet: Specific TaqMan microRNA assay and TaqMan Master Mix (Life Technologies, Inc., 4440040) were used to assess the expression of microRNAs. Submit your real-time PCR questions at http://www.lifetechnologies.com/asktaqmanAfter introducing MIQE guidelines, a set of standards for producing and publi paramount. Life Technologies has developed the TaqMan® Assays QPCR Guarantee to provide you with confidence in the data you gener-ate with a pre-designed TaqMan® Assay. Life Technologies guarantees that TaqMan® Gene Expression Assays will perform to your satisfaction. If you are not satisfied with the Scientific (formerly Life Technologies, Foster City, CA, USA).
av A NORRBELIUS — The results of the T. pallidum-specific PCR-assay showed that there was no cross-reactivity to other agents that are expected to be in ulcers polA-TaqMan prob 13. Gene quantification (2012) Real-time PCR handbook-Life Technologies. >.
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Single-base discrimination of TaqMan® MicroRNA Assays. Real-time TaqMan RT-PCR Assays for Selective Detection of Human Rhinovirus This invention relates to selective detection of human rhinovirus (HRV) in biological media. Specifically, this invention discloses a real-time TaqMan RT-PCR assay targeting the 5'-noncoding region of the HRV genome.
Centre for Ageing and Later Life), med inriktning på forskning om äldre och åldrande och KIPS Tackling ageing continence through theory, tools and technology S. A novel combination of Taqman rT-PCr and a suspension microarray assay for the detection and species identification of pestiviruses.
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Med FluoQube går det att typa HLA, RBC och HPA genom att kombinera FluoGene metoden (TaqMan® baserad SSP) med QPCR, för att få amplifiering och Intuitive analysis software for AriaMx qPCR data · Freeware · Access the data directly from your computer · Supports most assay types including SYBR®, TaqMan™, av R Maleckaite · 2019 · Citerat av 9 — Affiliations: Institute of Biosciences, Life Sciences Center, Vilnius The reaction mix (20 µl) consisted of 1X TaqMan Universal Master Mix II no Thermo Fisher Scientific, Inc.), 0.4X TaqMan Gene Expression assay Amplification was performed with Mx3005P™ qPCR System (Agilent Technologies, Inc., Genotypning: Genotypning av NPHS2-varianten rs61747728 kommer att utföras av TaqMan Allelic i realtid Diskrimineringsanalys (Life Technologies, CA, USA) TaqMan är ett varumärke som tillhör Roche Molecular Systems, Inc. Alla andra QXDx BCR‑ABL Primers & Probes. 1 Life Technologies Veriti Dx 96‑well.
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Life Technologies scientists have developed the most comprehensive set of real-time PCR assays available for gene expression, microRNA, noncoding RNA, protein analysis, mutation detection, copy number variation, and SNP genotyping. TaqMan® SNP Genotyping Assays from Life Technologies provide a highly flexible technology for detection of polymorphisms within any genome. TaqMan® Assays have the simplest workflow available and are the quickest way to generate genotyping data. Based on powerful TaqMan® probe and primer chemistry and designs, and coupled to dependable Applied Biosystems TaqMan OpenArray technology is an affordable real-time PCR–based solution for studying hundreds of samples per day, using the Applied Biosystems QuantStudio 12K Flex Real-Time PCR System with OpenArray Block. Each OpenArray plate can hold as many samples as 8 traditional 384-well plates.
This report describes stability and performance testing of three classes of assays (TaqMan® Gene Expression Assays, TaqMan ® MicroRNA Assays, and TaqMan SNP Genotyping Assays) after subjecting them to simulated summer ambient shipping multiplex PCR with TaqMan ® Assays, and covers the following subjects: • Guidance on optimizing multiplex PCR reactions • Information on using products from Life Technologies to facilitate the performance of multiplex PCR and decrease the amount of time required to perform optimization Applied Biosystems TaqMan Copy Number Assays use gold standard TaqMan MGB probe chemistry to evaluate the copy number of genomic DNA targets using Applied Biosystems real time PCR instruments and software TaqMan Copy Number Assays are run together with a TaqMan Copy Number Reference Assay in a duplex qPCR reaction the copy number assay detects the target sequence and the reference assay • TaqMan ® Non-coding RNA Assays – Target long non-coding RNA (ncRNA) in human, mouse, and rat species. These assays are designed to ncRNAs that are >60 nt in length. This protocol provides instructions for real-time reverse transcription-PCR (real-time RT-PCR) using TaqMan Gene Expression Assays and TaqMan Non-coding RNA Assays. Utility of TaqMan® hPSC ScorecardTM Assay in the Assessment of Functional Pluripotency of hPSCs Thermo Fisher Scientific • 5791 Van Allen Way • Carlsbad, CA 92008 • lifetechnologies.com Results Figure 1: Validating the TaqMan® hPSC ScorecardTM Panel COL2A1 TRPM8 BS2-1-C1 and BS2-1-C2 iPSC clones were derived from human fibroblasts Life Technologies Corporation | 6055 Sunol Blvd | Pleasanton, CA 94566 Products: TaqMan® Gene Expression Assays TaqMan® Gene Expression Assays, Primer‑Limited (PL) TaqMan® Non‑coding RNA Assay Custom Plus TaqMan® RNA Assay Custom PlusTaqMan® RNA Assay, Primer-Limited (PL) Custom TaqMan® Gene Expression Assays TaqMan® Gene Expression Assays Protocol 1. TaqMan® Gene Expression AssaysProtocol 2. For Research Use Only. Not for use in diagnostic procedures.Information in this document is subject to change without notice.APPLIED BIOSYSTEMS DISCLAIMS ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TOTHOSE OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE.